Evidence of uneven selective pressure on different subsets of the conserved human genome : implications for the significance of intronic and intergenic DNA

Peer reviewedPublisher PD

Understanding the Dynamics of Gene Regulatory Systems : Characterisation and Clinical Relevance of cis-Regulatory Polymorphisms

Peer reviewedPublisher PD

Using the CRISPR/Cas9 system to understand neuropeptide biology and regulation

Funding was provided by a Wellcome Trust ISSF starting grant (105625/Z/14/Z), Medical Research Scotland (PhD-719-2013), GW Pharmaceuticals (PhD-719-2013 - S.5242.001) and the BBSRC (BB/J012343/1).Peer reviewedPublisher PD

An Sp1 Modulated Regulatory Region Unique to Higher Primates Regulates Human Androgen Receptor Promoter Activity in Prostate Cancer Cells

Funding: This work was supported by the Chief Scientist’s Office (CSO) of the Scottish Government (http://www.cso.scot.nhs.uk/): CWH (CZB-4-477) and IH (ETM/382).Peer reviewedPublisher PD

Perspective: Quality Versus Quantity; Is It Important to Assess the Role of Enhancers in Complex Disease from an In Vivo Perspective?

AMcE was funded by BBSRC project grant (BB/N017544/1).Peer reviewedPublisher PD

Evidence for regulatory diversity and auto-regulation at the TAC1 locus in sensory neurones

Peer reviewedPublisher PD

Exploring the effects of polymorphisms on cis-regulatory signal transduction response

Peer reviewedPublisher PD

A hybrid slurry CFD model : Euler-Euler to Euler-Lagrange Tutorial

Detailed tutorial of hybrid model solver, carried out at Chalmers University, Gothenburg, Sweden

Investigating nucleation control in batch and flow using non-photochemical laser-induced nucleation

The practical application of non-photochemical laser-induced nucleation (NPLIN) to continuous flow was investigated. Supersaturated aqueous solutions were screened with a 5 ns pulsed laser (532 nm 44 MW cm-2) for NPLIN activity. Upon irradiation succinic acid nucleated at S20 = 4.3 and adipic acid at S20 = 2.0 – 3.0. NPLIN activity is reported for the first time in nicotinic acid (S20 = 2.6 – 3.0). No overall pattern was observed of chemical structure on NPLIN activity.From inorganic compounds similarly screened, ammonium chloride (S20 = 1.04 – 1.20) was identified as most suitable for further tests. It was shown to have an increase of NPLIN crystals with higher supersaturation from 13 at S = 1.038 to 252 at S = 1.135. A quadratic increase in number of crystals with increased laser power. The effects of NPLIN upon ammonium chloride are diminished upon filtration through a 0.2 μm poly (ether sulfone) filter, reducing the number of crystals from 350 to 10 per 70 mJ pulse (25 MW cm-2).The use of NPLIN in continuous flow was demonstrated from the first time. A S23 = 1.1 solution of aqueous ammonium chloride in flow produced crystals when irradiated by 10 pulses s-1 of a 1064 nm 6 ns laser. When the laser was stopped, crystals were no longer produced and the system returned to flowing supersaturated solution.Lab scale apparatus for continuous NPLIN experiments was developed. A design involving a re-dissolution step and loop flow was constructed for both laminar and slug-flow regimes. Nucleation of ammonium chloride (S = 1.1) was demonstrated in both systems. Repeatable NPLIN experiments were hindered by spontaneous nucleation. Spontaneous nucleation in flow was observed around areas where supersaturated solution passed from one component to another. Spontaneous nucleation was also observed upon cooling (25 to 10 °C). Filtration was observed to both suppress NPLIN and spontaneous nucleation in flow